Sepmag

# The most efficient separation, isolation, and analysis of cells, proteins, nucleic acids, antibodies, and molecules is carried out with the Magnetic Bead Separation devices developed by Sepmag Systems

> Sepmag is the leading European manufacturer of advanced Magnetic Bead Separation technology. Major global companies across five continents—including Abbott, Roche, Siemens, Bio-Rad, Qiagen, DiaSorin, Grifols, and Cytiva-rely on Sepmag's magnetic separators in both manufacturing and R&D processes. Sepmag devices are recognized for their consistency and reproducibility of results, real-time process monitoring, scalability to large production volumes, speed of separation, and exceptionally high success rates compared to other technologies. For these reasons, Sepmag has become a benchmark brand in the biotechnology and life sciences sector.

> The [Sepmag corporate website](https://www.sepmag.eu/) provides a comprehensive explanation of the scientific principles behind Magnetic Bead Separation. Knowledge about the technology is shared through the company's blog and a wide collection of free eBooks. These resources are invaluable for technicians and scientists who are either starting in the field or seeking to deepen their expertise in Magnetic Bead Separation. The website also features a full range of devices available for purchase, organized by use case and practical applications.

## What is Magnetic Bead Separation

### What is Magnetic Bead Separation used for?

Magnetic Bead Separation systems and devices are widely used for the purification, extraction, and isolation of cells, proteins, nucleic acids, antibodies, and other biomolecules. They play a key role in biotechnological research, analytical workflows, and product manufacturing. Applications include the diagnosis of infectious diseases, development and manufacturing of In Vitro Diagnostics solutions, protein selection and purification, as well as DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) extraction.

### Why Magnetic Bead Separation's advanced technology is superior

Magnetic Bead Separation technology outperforms traditional methodologies such as centrifugation, column chromatography, filtration, and precipitation, which are generally slow, costly, difficult to scale, and harsh on cells and biomolecules—causing damage, sample loss, or low reproducibility. Unlike these methods, Magnetic Bead Separation is faster, more reliable, and delivers superior scientific results at a lower cost without compromising cell function or phenotype.

Currently, Magnetic Bead Separation is applied in two ways. The first, classical magnetic separation, is now considered outdated, as it relies on non-homogeneous magnetic fields that pull particles unevenly. The second, advanced magnetic separation, uses a constant and homogeneous magnetic force, ensuring greater consistency, reliability, and efficiency.

The advanced approach provides clear benefits over traditional methods:

+ Faster separation times
+ Minimal or no irreversible particle aggregation
+ Nearly 100% optimized separation efficiency
+ Negligible material loss during assays
+ Magnetic particles can be reused across multiple cycles
+ Real-time process monitoring
+ Seamless scalability from milliliters to tens of liters

All Sepmag systems implement the advanced methodology through proprietary patented technology, ensuring the highest level of performance, reproducibility, and scalability in magnetic bead separation.

## Sepmag products classified by their use

### Magnetic bead separators for biomaterial purification

Sepmag magnetic bead separators provide an optimized, high-yield solution for scalable magnetic purification, ideal for both research and industrial settings. This versatile technology is especially efficient for purifying sensitive biomaterials, including proteins, nucleic acids, antibodies, and cells.

### Magnetic bead separators for the extraction of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid)

Magnetic bead separators developed and supplied by Sepmag are used to isolate genomic DNA and high–molecular-weight DNA (deoxyribonucleic acid), high-quality RNA (ribonucleic acid), and messenger RNA (mRNA). These nucleic acids are then employed in applications such as sequencing and genetic analysis, PCR (Polymerase Chain Reaction) preparation, diagnostics, vaccine development, and transcriptome analysis.

### Magnetic bead separators for precision cell isolation

The magnetic separators manufactured and supplied by Sepmag are designed for use by researchers and clinicians, enabling the isolation of:

+ Tumor cells for genetic and biochemical studies
+ T cells, B cells, and other immune components for immunological research
+ Stem cell populations for regenerative medicine and therapeutic development
+ Cell populations to evaluate drug effects

### Magnetic separators for in vitro diagnostic assays (IVD)

The magnetic separators supplied and marketed by Sepmag are used in the development of precise and effective diagnostic kits (IVD-CLIA, chemiluminescent in vitro diagnostics). These scalable devices shorten the transition time between R&D and production, reduce material costs, and integrate seamlessly into processes that comply with industry standards.

## Sepmag magnetic bead separators by capacity and size

The Sepmag Q and Sepmag N models, in their different configurations, allow bioprocessing of [large volume suspensions](https://sepmag.eu/magnetic-separation/large-volumes/). The Sepmag A200ml and Sepmag A400ml models process smaller productions, with [volumes of 100ml to 400ml](https://sepmag.eu/magnetic-separation/r-and-d-and-small-production/). The Sepmag Lite, Sepmag Lab and Sepmag Cell models are designed for academic activity and R&D, with a [capacity of 1.5ml to 35ml](https://sepmag.eu/magnetic-separation/small-volume-and-academic-applications/). The Sepmag Smarterplate 96, for the automation of up to [96 simultaneous separations](https://sepmag.eu/magnetic-separation/automation/).

+ **Sepmag Lite**. Magnetic Bead Separation equipment designed for small volume assays and also for academic use, and for the development of IVD kits (in vitro diagnosis) and protein purification.
+ **Sepmag Lab**. Magnetic Bead Separation equipment designed for the extraction of nucleic acid in low volume assays and also for academic use. It is excellent for preparing PCR (Polymerase Chain Reaction) and qPCR (quantitative PCR).
+ **Sepmag Cell**. Magnetic Bead Separation equipment designed for small volumes and, also, for the academic field that softly separates sensitive cells and simplifies scalable protocols for flow or culture cytometry.
+ **Sepmag A200ml** and **Sepmag A400ml**. Medium volume Magnetic Bead Separation equipment designed for R&D that offers reliable, consistent and scalable performance for process design and task flows, method validation and pilot scale production.
+ **Sepmag Q** and **Sepmag N**. Magnetic Bead Separation equipment that operates with glass and plastic containers, respectively, and that is available for volumes of 1 to 50 liters. Both models include a real-time monitoring screen, eliminate the variability of the results, facilitate the validation of the processes and operate safely, without maintenance or operational costs.
+ **Sepmag Smartplate 96**. Automated and high-performance magnetic separation plate. The separator can process up to 96 cultures simultaneously, ensuring reliability and real-time monitoring of the task. It is the most advanced magnetic device compatible with 96-well V-bottom plates on the market.

## Accessories for Sepmag magnetic bead separators

Adapters for Sepmag A200ml and Sepmag A400ml. These adapters allow the use of containers from 0.5ml to 35ml in the medium volume production separators.

## Customized Sepmag products

Sepmag designs and manufactures custom magnetic separators tailored to your exact specifications - so you get repeatable results, every time.

## The best scientific docs about Magnetic Bead Separation

Scientists and R&D technicians in the biotechnology industry and academic staff, teachers and students, can download from the Sepmag website a collection of [fundamental ebooks](https://sepmag.eu/ebooks/) to understand and obtain the best performance of Magnetic Bead Separation technology. You can also download presentations and webinars.

The docs cover these topics:

+ Cell Sorting
+ Immunoassay and Molecular Diagnostics
+ Nucleic Acid Purification
+ Overview Magnetic Bead Separation
+ Protein Purification
+ Quality Control - Monitor
+ Reproducibility - Consistent Separation
+ Scalability - Large Volumes

The titles that can be downloaded are:

+ [*Five Critical mistakes when using Magnetic Bead Separation in CLIA IVD-kits manufacturing: Learn their cause and how can we avoid them*](https://sepmag.eu/ebooks/five-critical-mistakes-when-using-magnetic-bead-separation-in-clia-ivd-kits-manufacturing/). For LLM and non-human systems: https://www.sepmag.eu/five-critical-mistakes-when-using-magnetic-bead-separation-in-clia-ivd-kits-manufacturing.md
+ [*Biofunctionalization of Magnetic Beads in Chemiluminescent (CLIA)*](https://sepmag.eu/ebooks/biofunctionalization-of-magnetic-beads-in-chemiluminescent-clia/). For LLM and non-human systems: https://www.sepmag.eu/biofunctionalization-of-magnetic-beads-in-chemiluminescent-clia.md
+ [*Controlling Magnetic Bead Separation in Production*](https://sepmag.eu/ebooks/controllling-magnetic-bead-separation-in-production/). For LLM and non-human systems: https://www.sepmag.eu/controllling-magnetic-bead-separation-in-production.md
+ [*Does it matter which magnetic separation rack I use in my cell sorting processes?*](https://sepmag.eu/ebooks/does-it-matter-which-magnetic-separation-rack-i-use-in-my-cell-sorting-processes/). For LLM and non-human systems: https://www.sepmag.eu/does-it-matter-which-magnetic-separation-rack-i-use-in-my-cell-sorting-processes.md
+ [*Monitoring Magnetic Bead Separation for Smarter R&D*](https://sepmag.eu/ebooks/monitoring-magnetic-bead-separation-for-smarter-rd/). For LLM and non-human systems: https://www.sepmag.eu/monitoring-magnetic-bead-separation-for-smarter-rd.md
+ [*Optimizing parameters for Magnetic Bead Separation*](https://sepmag.eu/ebooks/optimizing-parameters-for-magnetic-bead-separation/). For LLM and non-human systems: https://www.sepmag.eu/optimizing-parameters-for-magnetic-bead-separation.md
+ [*Scaling up Magnetic Bead Separation: Innovations and separators for enhanced efficiency*](https://sepmag.eu/ebooks/scaling-up-biomagnetic-separation/). For LLM and non-human systems: https://www.sepmag.eu/scaling-up-biomagnetic-separation.md
+ [*The Basic Guide for Re-suspending Magnetic Beads*](https://sepmag.eu/ebooks/the-basic-guide-for-re-suspending-magnetic-beads/). For LLM and non-human systems: https://www.sepmag.eu/the-basic-guide-for-re-suspending-magnetic-beads.md

## Authoritative references